Chromatin higher order structure: Opening up chromatin for transcription
نویسندگان
چکیده
منابع مشابه
Methods for identifying higher-order chromatin structure.
Eukaryotic genomic DNA is combined with histones, nonhistone proteins, and RNA to form chromatin, which is extensively packaged hierarchically to fit inside a cell's nucleus. The nucleosome-comprising a histone octamer with 147 base pairs of DNA wrapped around it-is the initial level and the repeating unit of chromatin packaging, which electron microscopy first made visible to the human eye as ...
متن کاملChromatin higher-order structure and dynamics.
The primary role of the nucleus as an information storage, retrieval, and replication site requires the physical organization and compaction of meters of DNA. Although it has been clear for many years that nucleosomes constitute the first level of chromatin compaction, this contributes a relatively small fraction of the condensation needed to fit the typical genome into an interphase nucleus or...
متن کاملDistinctive higher-order chromatin structure at mammalian centromeres.
The structure of the higher-order chromatin fiber has not been defined in detail. We have used a novel approach based on sucrose gradient centrifugation to compare the conformation of centromeric satellite DNA-containing higher-order chromatin fibers with bulk chromatin fibers obtained from the same mouse fibroblast cells. Our data show that chromatin fibers derived from the centromeric domain ...
متن کاملHigher-order chromatin structure: bridging physics and biology.
Advances in microscopy and genomic techniques have provided new insight into spatial chromatin organization inside of the nucleus. In particular, chromosome conformation capture data has highlighted the relevance of polymer physics for high-order chromatin organization. In this context, we review basic polymer states, discuss how an appropriate polymer model can be determined from experimental ...
متن کاملOn key modulators of higher-order chromatin structure
The billions of DNA bases that comprise mammalian genomes encode the instructions to assemble complex organisms consisting of hundreds of different cell types. Apart from the challenge of packaging this genetic material into a nuclear space that is six orders of magnitude smaller than its linear sequence, the molecules need to be organised in such a way as to ensure the coordinated activity of ...
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ژورنال
عنوان ژورنال: Briefings in Functional Genomics and Proteomics
سال: 2004
ISSN: 1473-9550,1477-4062
DOI: 10.1093/bfgp/2.4.334